{"id":2208,"date":"2022-04-28T08:52:30","date_gmt":"2022-04-28T08:52:30","guid":{"rendered":"https:\/\/biolabtek.vn\/?p=2208"},"modified":"2023-01-09T03:15:49","modified_gmt":"2023-01-09T03:15:49","slug":"tieu-chuan-quoc-gia-tcvn-8400-412019-benh-dong-vat-quy-trinh-chan-doan-phan-41-benh-dich-ta-lon-chau-phi-animal-disease-diagnostic-procedure-part-41-african-swine-fever","status":"publish","type":"post","link":"https:\/\/biolabtek.vn\/?p=2208","title":{"rendered":"TI\u00caU CHU\u1ea8N QU\u1ed0C GIA TCVN 8400-41:2019 B\u1ec6NH \u0110\u1ed8NG V\u1eacT – QUY TR\u00ccNH CH\u1ea8N \u0110O\u00c1N – PH\u1ea6N 41: B\u1ec6NH D\u1ecaCH T\u1ea2 L\u1ee2N CH\u00c2U PHI Animal disease – Diagnostic procedure Part 41: African Swine Fever"},"content":{"rendered":"

Ph\u1ee5 l\u1ee5c E<\/strong><\/p>\n

(Tham kh\u1ea3o)<\/p>\n

Ph\u01b0\u01a1ng ph\u00e1p ELISA ph\u00e1t hi\u1ec7n kh\u00e1ng th\u1ec3 ASFV<\/strong><\/p>\n

S\u1eed d\u1ee5ng k\u00edt th\u01b0\u01a1ng m\u1ea1i Ingezim PPA compac 11.PPA.K3 (\u00a0https:\/\/biolabtek.vn\/?product=ingezim-ppa-compac)<\/a><\/p>\n

E.1 \u00a0Chu\u1ea9n b\u1ecb nguy\u00ean li\u1ec7u<\/strong><\/p>\n

– T\u1ea5t c\u1ea3 c\u00e1c nguy\u00ean li\u1ec7u (ngo\u1ea1i tr\u1eeb Conjugate) \u0111\u1ec3 30 ph\u00fat \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (20 \u00b0C \u0111\u1ebfn 25 \u00b0C) tr\u01b0\u1edbc khi ti\u1ebfn h\u00e0nh ph\u1ea3n \u1ee9ng.<\/p>\n

– Pha lo\u00e3ng dung d\u1ecbch n\u01b0\u1edbc r\u1eeda 40 X: 40 ml dung d\u1ecbch n\u01b0\u1edbc r\u1eeda \u0111\u1eadm \u0111\u1eb7c v\u1edbi 960 ml n\u01b0\u1edbc kh\u1eed ion.<\/p>\n

E.2 \u00a0C\u00e1ch ti\u1ebfn h\u00e0nh<\/strong><\/p>\n

    \n
  1. Nh\u1ecf 50 \u03bcl Dilution Buffer v\u00e0o c\u00e1c gi\u1ebfng:<\/li>\n<\/ol>\n

    Nh\u1ecf 50 \u03bcl huy\u1ebft thanh \u0111\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng v\u00e0o 2 gi\u1ebfng (v\u00ed d\u1ee5: A1 v\u00e0 B1)<\/p>\n

    Nh\u1ecf 50 \u03bcl huy\u1ebft thanh \u0111\u1ed1i ch\u1ee9ng \u00e2m v\u00e0o 2 gi\u1ebfng (v\u00ed d\u1ee5: A2 v\u00e0 B2)<\/p>\n

    Nh\u1ecf 50 \u03bcl huy\u1ebft thanh c\u1ea7n ki\u1ec3m tra v\u00e0o c\u00e1c gi\u1ebfng c\u00f2n l\u1ea1i.<\/p>\n

      \n
    1. D\u00e1n \u0111\u0129a l\u1ea1i v\u00e0 \u1ee7 \u1edf nhi\u1ec7t \u0111\u1ed9 36 \u00b0C (\u00b1 1 \u00b0C) trong 1 gi\u1edd \u00b1 5 ph\u00fat ho\u1eb7c qua \u0111\u00eam (t\u1eeb 16 gi\u1edd \u0111\u1ebfn 20 gi\u1edd) \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng t\u1eeb 20 \u00b0C \u0111\u1ebfn 25 \u00b0C.<\/li>\n
    2. \u0110\u1ed5 b\u1ecf dung d\u1ecbch trong \u0111\u0129a v\u00e0o b\u00ecnh ch\u1ea5t th\u1ea3i ch\u1ee9a dung d\u1ecbch NaOH; r\u1eeda \u0111\u0129a b\u1eb1ng c\u00e1ch th\u00eam 300 \u03bcl dung d\u1ecbch n\u01b0\u1edbc r\u1eeda \u0111\u00e3 pha \u1edf 1 v\u00e0o c\u00e1c gi\u1ebfng, r\u1ed3i \u0111\u1ed5 b\u1ecf. L\u1eb7p l\u1ea1i 4 l\u1ea7n.<\/li>\n
    3. Chu\u1ea9n b\u1ecb Conjugate 1X b\u1eb1ng c\u00e1ch pha lo\u00e3ng conjugate 100 X (v\u00ed d\u1ee5: 110 \u03bcl conjugate v\u00e0o 11 ml Dilution), l\u1eafc nh\u1eb9 tr\u01b0\u1edbc khi d\u00f9ng.<\/li>\n
    4. Nh\u1ecf 100 \u03bcl dung d\u1ecbch Conjugate 1 X v\u00e0o t\u1ea5t c\u1ea3 c\u00e1c gi\u1ebfng.<\/li>\n
    5. D\u00e1n \u0111\u0129a v\u00e0 \u1ee7 \u1edf nhi\u1ec7t \u0111\u1ed9 36 \u00b0C (\u00b1 1 \u00b0C) trong 30 ph\u00fat.<\/li>\n
    6. R\u1eeda \u0111\u0129a 5 l\u1ea7n v\u1edbi dung d\u1ecbch r\u1eeda nh\u01b0 b\u01b0\u1edbc 3.<\/li>\n
    7. Nh\u1ecf 100 \u03bcl dung d\u1ecbch Subtrate v\u00e0o c\u00e1c gi\u1ebfng. \u0110\u1eb7t \u0111\u0129a \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng t\u1eeb 20 \u00b0C \u0111\u1ebfn 25 \u00b0C trong v\u00f2ng 15 ph\u00fat.<\/li>\n
    8. Th\u00eam 100 \u03bcl dung d\u1ecbch stop Solution v\u00e0o c\u00e1c gi\u1ebfng \u0111\u1ec3 d\u1eebng ph\u1ea3n \u1ee9ng.<\/li>\n
    9. \u0110o gi\u00e1 tr\u1ecb OD (m\u1eadt \u0111\u1ed9 quang h\u1ecdc) b\u1eb1ng m\u00e1y \u0111\u1ecdc ELISA (m\u1ee5c 5.3.1) \u1edf b\u01b0\u1edbc s\u00f3ng 450 nm trong v\u00f2ng 5 ph\u00fat sau khi d\u1eebng ph\u1ea3n \u1ee9ng.<\/li>\n<\/ol>\n

      E3. \u00a0\u0110\u1ecdc k\u1ebft qu\u1ea3<\/strong><\/p>\n

      – \u0110i\u1ec1u ki\u1ec7n ph\u1ea3n \u1ee9ng \u0111\u01b0\u1ee3c c\u00f4ng nh\u1eadn khi: NC\/PC \u2265 4, trong \u0111\u00f3:<\/p>\n

      NC: M\u1eadt \u0111\u1ed9 quang h\u1ecdc (OD) trung b\u00ecnh c\u1ee7a \u0111\u1ed1i ch\u1ee9ng \u00e2m<\/p>\n

      PC: M\u1eadt \u0111\u1ed9 quang h\u1ecdc (OD) trung b\u00ecnh c\u1ee7a \u0111\u1ed1i ch\u1ee9ng d\u01b0\u01a1ng<\/p>\n

      – \u0110\u00e1nh gi\u00e1 k\u1ebft qu\u1ea3 d\u1ef1a tr\u00ean X % c\u1ee7a m\u1eabu theo c\u00f4ng th\u1ee9c sau:<\/p>\n

      X% = [(NC – Sample OD)\/ (NC-PC)] x 100 %<\/p>\n

      + M\u1eabu d\u01b0\u01a1ng t\u00ednh khi: X % \u2265 50 %<\/p>\n

      + Mau \u00e2m t\u00ednh khi: X % \u2264 40 %<\/p>\n

      + M\u1eabu c\u00f3 gi\u00e1 tr\u1ecb 40 % < X % < 50 % \u0111\u01b0\u1ee3c cho l\u00e0 nghi ng\u1edd<\/p>\n

      Ngu\u1ed3n:<\/p>\n